MCQs BIOCHEMISTRY: Chromatography

1.
Thin layer chromatography is

A. partition chromatography
B. electrical mobility of ionic species
C. adsorption chromatography
D. none of the above
Answer & Explanation
Answer: Option C

2.
In gas chromatography, the basis for separation of the components of the volatile material is the difference in

A. partition coefficients
B. conductivity
C. molecular weight
D. molarity
Answer & Explanation
Answer: Option A

3.
In reverse phase chromatography, the stationary phase is made

A. non-polar
B. polar
C. either non-polar or polar
D. none of these
Answer & Explanation
Answer: Option A

4.
Ion exchange chromatography is based on the

A. electrostatic attraction
B. electrical mobility of ionic species
C. adsorption chromatography
D. partition chromatography
Answer & Explanation
Answer: Option A

5.
The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

A. V = V0 + kDVi
B. V = V0/Vi
C. V = V0 - kDVi
D. V/V0 = kDVi
Answer & Explanation
Answer: Option A

6.
A combination of paper chromatography and electrophoresis involves

A. partition chromatography
B. electrical mobility of the ionic species
C. both (a) and (b)
D. none of these
Answer & Explanation
Answer: Option C